Cozap: Destaining gels without changing solutions. How do you remove the Coomassie blue from your acrylamide gel destain solution? Add more solution? Toss in a piece of foam? Maybe you rely on a mushy ...

3| Pool the supernatants (∼30 ml) and add 30 μg of the appropriate antibody (that will precipitate ... 8| Visualize the protein bands by staining with Coomassie blue, excise the band of ...