CBB染色の原理とプロトコル(方法)・組成【SDS-PAGE】
CBB（Coomassie Brilliant Blue）染色は、ドイツの研究者であったVolker Neuhoffによって最初に報告されました。Coomassieの名前は、1896年にイギリスがアフリカのKumasiを占 … 詳細
CBBとタンパク質の結合様式は、CBBのR-250とG-250側のスルホ（Sulfonic acid）基とタンパク質側のアミノ（Amino）基によるイオン結合、およびファンデルワールス力による結合です。どちらも非共有結合です。 CBB染色液中にSDS-PAGEゲルを浸すこと … 詳細
SDS-PAGE後のゲルを染色してバンドを確認する方法には、銀染色法もあります。 銀染色の方が感度が高く、ゲル中の数ナノグラムのタンパク質を検出 … 詳細
- CBB染色に必要な試薬・ラボウェア
- 1. クマシーブリリアントブルー R-250（Coomassie Brilliant Blue R-250, CA…
- CBB染色液とCBB脱色液の作製（組成;作り方）
- 1. CBB染色液 100mL 250m… 詳細
- 1. CONDUCTSCIENCE, Coomassie Brilliant Blue Stain Protocol.
- 2. He, F. (2011). Coomassie Blue Staining. Bio-101: e78. DOI: 10.21769/BioProtoc.78.
- 3. ProtocolsOn… 詳細
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To prepare 1 l of a staining solution, dissolve 1 g of Coomassie R250 to 300 ml of methanol. Then add 650 ml of MQ water and 50 ml of acetic acid. Stir the solution on a magnetic stirrer for 2 h. …
2019年2月1日 · 5.1.1 Staining Solution (Developer) 5.1.1.1 Place 0.44 g of Coomassie Brilliant Blue and 200 mL of methanol in a large glass beaker with a magnetic stir bar and stir. 5.1.1.2 …
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Coomassi Blue Staining | Thermo Fisher Scientific - US
Prepare the staining solution containing 0.1% Coomassie R-250 in 40% ethanol, 10% acetic acid. After electrophoresis, incubate 1 or 2 gels in a staining container containing 100 ml Coomassie …
Protocol for Coomassie Staining - AAT Bioquest
Recent advances in Coomassie staining have focused on enhancing sensitivity and simplifying the process of staining the protein gels. Our ProLite™ FAST Blue Protein Gel Stain is a ready-to …
Stain solution preparation: Add 100mL of glacial acetic acid to 450mL ultrapure water. Dissolve the 3g of Coomassie Dye in 450mL methanol. Filter the solution before use. Gel may be …
Blue Fast Staining Solution (Coomassie Blue Fast Staining Solution) is based on Coomassie Blue G250 as the dye, and can be used for non-contaminating, rapid and highly sensitive staining of …
Remove the gel from the electrophoresis chamber and place enough 0.5% Coomassie Blue G-250 (prepared in 50% methanol/ 10% acetic acid) to cover the gel. Use freshly washed labware that …
Coomassie Brilliant Blue staining solution - CSH Protocols
Dissolve 1 g of Coomassie Brilliant Blue (Bio-Rad) in 1 liter of the following solution: Methanol (50% [v/v]) Glacial acetic acid (10% [v/v])
Evaluation of Coomassie blue staining of the acrosome of ...
2006年2月1日 · Acrosomal staining by Coomassie blue stain is an effective and inexpensive method for evaluating the acrosome of spermatozoa from humans, cattle, swine, rabbits, …
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